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Part:BBa_K4829006:Experience

Designed by: Aditya Kamath Ammembal   Group: iGEM23_IISc-Bengaluru   (2023-10-04)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4829006

We have used this part in BBa K4829003, and we noticed expression in Hela cells, 6hrs post-transfection. We used the constructs, with 2ug of mRNA mixed with 2ul of Lipofectamine MessengerMAX, in each well of a 12-well plate, each well having 0.5 million cells. The protein was noticed in the supernatant. The cell lysate was not loaded, and the control was clear media. The blot results were as follows:

  • hela-blot.png
 Figure 1. Expression of the protein noted in the supernatant 6 hours post transfection ]]


The 2 lanes beside the bands are controls, the blot was done in duplicates. There was an unfortunate amount of background, yet the band at 31kDa is quite clear.

User Reviews

UNIQ21046e8b80bd3cee-partinfo-00000000-QINU UNIQ21046e8b80bd3cee-partinfo-00000001-QINU